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The CytoFLEX flow cytometer uses simplified light path, relying on a single bandpass filter versus a combination of dichroic and bandpass filters specifically ordered. In this part of our Magic Inside video series, you will be transported inside of the instrument and follow along the light path, learning how the reduction in the number of light interactions contributes to the exquisite Instructional video for starting up BYU's CytoFlex CytometerNarrated by Dr. Donald BreakwellFilmed and edited by Gabriel Goulding CYTOFLEX QUICK INTRO v190926 See below for A) icon for Acquisition or Analysis, B) Status bar, C) Acquisition screen, D) Plot area icons, E) Software commands tree STEP Description 1. Start Up - Information seen on wall - Start up will take 7 minutes - Once started up make sure you are in Acquisition CytoFLEX LX Flow Cytometer UV-Violet-Blue-Yellow Green-Red-Infrared (U-V-B-Y-R-I) Series TECHNICAL SPECIFICATION OPTICS EXCITATION OPTICS The instrument has the capacity for 23 parameters, including 21 for fluorescence detection. The fully activated instrument includes three fluorescent channels from the 355 nm (UV) laser, five from the 405 nm About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators DxFLEX is a new clinical flow cytometry platform derived from the successful CytoFLEX. The advanced sensitivity and intuitive software DxFLEX makes flow cytometry routine for both novice and expert flow cytometry technicians and promotes standardization. Functionality in the autoloader facilitates accurate results and sample tracking. The CytoFLEX Flow Cytometer provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation.

The advanced sensitivity and intuitive software DxFLEX makes flow cytometry routine for both novice and expert flow cytometry technicians and promotes standardization. Functionality in the autoloader facilitates accurate results and sample tracking. The CytoFLEX Flow Cytometer provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation. The system can be configured for the needs of your laboratory, whether it is routine low complexity analysis, high complexity analysis, or analysis that pushes the boundaries for flow cytometry.

Flow Cytometric Analysis of Extracellular Vesicles from Cell

• Fill with Cytoflex sheath fluid. Stanford Cytoflex User Guide 111115 Starting up 1. Check the sheath fluid level every time you use the cytometer.

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A detailed description of how to perform this operation can be found in the CytoFlex Operation Manual. Briefly, you will access the Violet laser optical filters inside the instrument, place the Violet 405nm filter in position 2, the Violet 450, 525, 610 and 660 filters in positions 3-6, and an unused filter in position 1. MANUAL TUBE/PLATE SWITCH Part Number, Description C16574, CytoFLEX Plate Loader DW C02396, CytoFLEX Plate Loader with Mode Control Upgrade Kit B63215, CytoFLEX Plate Loader Tube or Plate Sample Injection Mode Sample Injection Mode can be changed by using the Sample Injection Mode Control Switch. Sample Injection Mode can be changed by using MANUAL TUBE/PLATE SWITCH Part Number, Description C16574, CytoFLEX Plate Loader DW C02396, CytoFLEX Plate Loader with Mode Control Upgrade Kit B63215, CytoFLEX Plate Loader Tube or Plate Sample Injection Mode Sample Injection Mode can be changed by using the Sample Injection Mode Control Switch. Sample Injection Mode can be changed by using CytoFlex LX A and B The facility has two identically configured CytoFLEX LX analyzers. Each is a five laser system capable of detecting in 19 different channels, please see the configuration below for details. CytoFLEX delivers and surpasses capabilities expected in top tier analyzers, with excellent performance and nanoparticle resolution.

Replenish fluids if needed as follows: • Unscrew and remove the cap from the sheath tank. • Fill with Cytoflex sheath fluid. Stanford Cytoflex User Guide 111115 Starting up 1. Check the sheath fluid level every time you use the cytometer. This ensures that you do not run out of sheath fluid during an experiment. Replenish fluids if needed as follows: • Unscrew and remove the cap from the sheath tank.
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Turn on machine 3. Sign into computer and start CytExpert Software 4. Select Cytometer sample injection to Semi-Automatic (Tubes) or plates 5.

The high-performance CytoFLEX system offers scalable detection capabilities for up-to three lasers and 13-color research flow cytometry right on your benchtop. Simplifying High Complexity Flow Cytometry Experiments: a Case Study Using an Analysis of T Cell Populations in Human Blood T Cell Biology T cells form an integral part of the human immune system by fighting off infection and eliminating transformed cells. CytoFLEX S Systems are available in preset configurations with up to four lasers, including 561 nm, 375 nm or lasers in the original palette and up to 13 channels for fluorescence detection. Just like the CytoFLEX, the CytoFLEX S utilizes the same advanced technology to deliver superior sensitivity and resolution, comparable to or even exceeding instruments four times its size.
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The high-performance CytoFLEX system offers scalable detection capabilities for up-to three lasers and 13-color research flow cytometry right on your benchtop. The CytoFLEX S Flow Cytometer series is an expansion of the CytoFLEX Platform. The CytoFLEX Flow Cytometer offers three laser options, 488 nm, 638 nm, and 405 nm. The CytoFLEX S Flow Cytometer platform expands your research possibilities, with a fourth laser option, multiple configurations and unique filter sets for your science.

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14)Close soware. 15)Turn oﬀ the instrument power and leave computer ON. Changing to plate reader from semi-automac tube loader Stanford Cytoflex HTS User Guide 11232015 Starting up 1. Check the sheath fluid level every time you use the cytometer. This ensures that you do not run out of sheath fluid during an experiment. Replenish fluids if needed as follows: • Unscrew and remove the cap from the sheath tank.

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Thomas Andl, PhD. Core Lab, Research Biomolecular Annex. May 15, 2020 From the Cytometer menu, select System Startup Program and follow the instructions. Note: After initializing the System Startup Program, place a 二、 CytoFLEX 使用Channels 及對應螢光染劑 5 abnormally. For example, the well is skipped or the acquisition is manually stopped. MANUAL.

15)Turn oﬀ the instrument power and leave computer ON. Changing to plate reader from semi-automac tube loader Stanford Cytoflex HTS User Guide 11232015 Starting up 1.